DisneyQuest at Downtown Disney will close next year to make way for the NBA Experience.
The 100,000-square-foot indoor virtual theme park and hands-on entertainment center with video and virtual games first opened in 1998 at the entertainment district's West Side.
Ed Chambers, president of the Service Trades Council coalition of Disney unions, said new jobs will be available for DisneyQuest workers.
Disney earlier this month announced it was planning a new NBA-themed attraction that will include high-tech experiences, a restaurant and a store. Downtown Disney is currently undergoing an expansion, and it will be renamed Disney Springs.